17, 18, 19 It is estimated that about 85% of the human genome is transcribed into RNA, 20 with more than 80% of the genome estimated to be biologically and functionally relevant, 21 however, only 1.5 percent of the genome encodes proteins. RNA plays an essential role in various important biological processes by folding and sustaining a three-dimensional (3D) structure in order to perform functions such as catalysis and gene regulation. 13 Readers interested in technical developments and current challenges in SPA are directed to a number of comprehensive reviews for further details. Whereas the above features make SPA particularly useful to determine structures of larger proteins and protein complexes (>200 kDa), additional technical advances, especially the development of Volta phase plate (VPP), 12 have pushed the limit of protein molecular weight to as low as 52 kDa for structure determination by SPA. 9 Because cryo-EM SPA is featured by minimal amount of specimen under near-native crystal-free condition, automated data collection with continually increasing throughput, 10 and comprehensive data processing pipelines capable of resolving structural heterogeneity, 11 it has become a widely adopted structural biology technique for structural biologists whose portfolio used to have only X-ray crystallography and NMR. 3, 4, 5 Aside from single-particle analysis (SPA) that has gained tremendous popularity, other cryo-EM approaches have also benefited from the revolution, such as cryo-electron tomography (cryo-ET), 6, 7 micro-electron diffraction (MicroED) 8, and cryo-scanning transmission electron microscopy (cryo-STEM). 1, 2 More recently, several studies have achieved atomic-resolution cryo-EM reconstruction using either the next-generation hardware or the most up-to-date commercially available setup. Less experienced users can quickly get familiarized with SlimBoat's features.The recent resolution revolution triggered by the development of direct electron detectors and other technical advances has allowed cryo-EM to break the previous resolution barrier(s) and led to exponential growth of near-atomic cryo-EM structures. We have not come across any problems during our evaluation SlimBoat did not freeze, crash or pop up error dialogs. The web browser has a good navigation speed and response time, uses a moderate amount of system resources, and includes user documentation. browser history, cookies, icon cache), customize the weather display in the status bar, just to name a few. restore last open sites, open a specific page or group), make the tool clean items (e.g. From the 'Tools' menu, you have direct access to some Facebook functions (share your status, page or selected text).īut the tool also lets you share a page on Twitter, Google+, StumbleUpon,, , Gmail, Hotmail, or Yahoo Email, from the same menu.Īdditional features of SlimBoat let you manage downloads, enable a web inspector, download all content from the current page, enable ad and popup blockers, customize aliases for URLs and assign site names.īy accessing the 'Settings' panel, you can configure startup options (e.g. SlimBoat packs an autofill form feature - with the best match, saved forms, the current identity or identities. So, you can add and manage bookmarks, translate the current page or selected text, add and organize groups, as well as view and access the thumbnails of the last opened pages. The interface of the tool is clean and familiar. SlimBoat is yet another multi-tabbed web browser that integrates some powerful features which focus on speed, stability and plug-ins.
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